KMID : 1134820110400050642
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Journal of the Korean Society of Food Science and Nutrition 2011 Volume.40 No. 5 p.642 ~ p.648
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Evaluation of Biological Activity in Pepper (Capsicum annuum L.) Breeding Lines
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Jung Mi-Ri
Hwang Young Kim Hye-Young Cho Myeong-Cheoul Hwang In-Guk Yoo Seon-mi Jeong Heon-Sang Lee Jun-Soo
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Abstract
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The purpose of this study was to determine the capsaicinoid and antioxidant compounds and to evaluate thebiological activity of 40 different pepper (Capsicum annuum L.) breeding lines. Capsaicinoid and ascorbic acidcontent were measured with HPLC. Total polyphenolic and flavonoid contents and antioxidant activity weremeasured with spectrophotometric methods. The antiproliferative qualities of the samples against human breasttumor cells (MCF7) were assessed with a MTT assay. The nitric oxide content in the culture media was measuredto evaluate the anti-inflammatory qualities of the samples using Raw264.7 macrophages. The capsaicinoid, ascorbicacid, polyphenolic and flavonoid content of the 40 pepper breeding lines were 0.1¢¦204.2 §·/100 g (dryweight basis, DWB), 279.1¢¦1695.5 §·/g (DWB), 2.6¢¦10.2 §·/g (DWB), and 1.4¢¦5.7 §·/g (DWB), respectively.The highest antioxidant, antiproliferative, and anti-inflammatory qualities were found in breeding line numbers2500, 3201, and 3232, respectively. This study provides basic information useful to plant breeders and biotechnologistswho are planning to breed pepper genotypes with high phytochemical content.
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KEYWORD
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pepper, breeding line, capsaicinoid, antioxidant activity, anti-inflammatory activity
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